Conformational flexibility of the dengue virus RNA - dependent RNA 1 polymerase revealed by a complex with an inhibitor 2 3
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چکیده
Conformational flexibility of the dengue virus RNA-dependent RNA 1 polymerase revealed by a complex with an inhibitor 2 3 Christian G. Noble, Siew Pheng Lim, Yen-Liang Chen, Chong Wai Liew, Lijian 4 Yap, Julien Lescar *, and Pei-Yong Shi* 5 6 Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01 Chromos, 7 School of Biological Sciences, Nanyang Technological University, Singapore 8 CNRS, AFMB UMR 7257, 13288, Marseille, France 9 10 11 12 *Corresponding authors: 13 Julien Lescar: School of Biological Sciences, Nanyang Technological University; 14 Phone: +65 65869706; Fax: +65; E-mail: [email protected] 15 Pei-Yong Shi: 10 Biopolis Road, 05-01, Chromos, Singapore 138670; Phone: +65 16 67222909; Fax: +65 67222916; E-mail: [email protected] 17 18 Running title: Dengue virus polymerase structure 19 20
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Conformational flexibility of the Dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor.
We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational c...
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